It did not say which countries would be affected, but said the positions would be closed in the next few weeks.
Boss Andy Jassy said it was a difficult decision but it would be best for the company in the long term.
He said that because of the uncertain economy in which we reside and the uncertainty that exists in the near future, we have chosen to be more streamlined in our costs and headcount.
Its sales have slowed as consumers spend less due to the cost of living crisis.
Other companies, including Google and Facebook-owned Meta, have been struggling with how to balance cost-cutting measures with the need to remain competitive.
Jassy said it is never easy to lose employees and that they are impacted by these reductions and to thank you for the work you have done on behalf of customers and the company. It came days after Emmett Shear announced he would step down as Twitch's chief executive officer after 16 years in office.