The Soft drink company confirmed on Friday it will not be renewing its deal with the All England Lawn Tennis Club.
Robinsons Lemon Barley Water was first officially introduced in bottles on the steps of the umpires' seats at Wimbledon.
Britain's Fred Perry and American Helen Moody won the singles titles that year, and squash became the official soft drink provider for the championships.
Prior to the deal, Smedley Hodgson placed Robinsons Barley Water in players' dressing rooms during the 1930s after combining barley crystals with real lemon juice and sugar, creating a refreshing and hydrating drink.
A spokesman for Britvic, which owns several drinks brands including Robinsons, said it was very proud to have been a prominent partner in this historic tournament for so many years and the wider role that we have played in the game of tennis in the UK. Britvic announced last month that Robinsons would be a three-year deal as sponsor of The Hundred, cricket's 100 ball competition, for the 2022 season.
Two decades after making its debut on centre court, Robinsons Lemon Barley Water was appointed by The Queen in 1955 with a royal warrant.