It didn't say which countries would be affected but said the positions would be closed in the next few weeks.
Boss Andy Jassy said it was a difficult decision but it would be best for the company in the long term.
He said that due to the uncertain economy in which we reside, and the uncertainty that exists in the near future, we have chosen to be more streamlined in our costs and headcount.
Its sales have slowed down recently as consumers spend less due to the cost of living crisis.
Other companies, including Google and Facebook-owned Meta, have been grappling with how to balance cost-cutting measures with the need to remain competitive.
"It is never easy to lose employees," Jassy said. "To those who are ultimately affected by these reductions, I want to thank you for the work you have done on behalf of customers and the company." It comes days after Emmett Shear announced he would step down as Twitch's chief executive officer after 16 years in office.